International Journal of Environmental Science and Development

Abstract—Utilizing whole-cell biocatalyst is a potential way to reduce catalyst cost in biodiesel production using lipase as catalyst. Whole-cell of Rhizopus oryzae was cultivated by one-step and two-step method and was immobilized on Biomass Support Particles (BSPs) and chitosan-TPP bead. Immobilized whole-cells on BSPs produce 11% (one-step) and 12% (two-step) FAME yield. While, FAME yield produced by immobilized whole-cell in chitosan-TPP beads are 23% (one-step) and 22% (two-step). Kinetic model based Michaelis-Menten used was found to fit fairly the substrate and product concentration profile. Value of Km and Vmax for R. oryzae whole-cell immobilized on BSP are 4 mole L^-1, 0.05 mole L^-1 h^-1 (one-step) and 3 mole L-1, 0.04 mol L^-1 h^-1 (two-step). While, for immobilized whole-cell in chitosan-TPP bead, the values are 0.3 mole L^-1, 0.01 mole L^-1 h^-1 and 0.2 mole L^-1, 0.008 mole L^-1 h^-1 for single-step and two-step respectively.

Index Terms—Biodiesel, immobilization, Michaelis-Menten, whole-cell.

H. Hermansyah, M. B. Faiz, and R. Arbianti are with Universitas Indonesia, Depok 16424, Indonesia (e-mail: heri@che.ui.ac.id, merisa.faiz@ui.ac.id, arbianti@che.ui.ac.id).

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Cite:H. Hermansyah, M. B. Faiz, and R. Arbianti, "Kinetic Study of Immobilized Whole-Cell Lipase Rhizopus Oryzae as Biocatalyst in Biodiesel Synthesis through Non-Alcohol Route," International Journal of Environmental Science and Development vol. 6, no. 6, pp. 439-444, 2015.