International Journal of Environmental Science and Development

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Volume 4 Number 4 (Aug. 2013)

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IJESD 2013 Vol.4(4): 360-364 ISSN: 2010-0264
DOI: 10.7763/IJESD.2013.V4.371

Genetically Engineered Bacteria Immobilized in Alginate as an Option of Cyanotoxins Removal

Dariusz Dziga, Magdalena Sworzen, Benedykt Wladyka, and Marcin Wasylewski
Abstract—Microcystins (MCs), cyclic heptapeptides produced by cyanobacteria constitute a significant risk to humans. Several bacteria with MCs degradation capability are known, however their practical usage is questionable due to low MC elimination efficiency. As an alternative bacteria with significantly enhanced activity toward these hepatotoxins may be constructed. We have recently presented successful expression of the MlrA enzyme involved in MC biodegradation in E. coli BL21 strain. In this paper preliminary experiments indicating the usefulness of such modified bacteria have been showed. The MC degradation efficiency was monitored by the HPLC detection of linear MC-LR. Immobilization procedure involved the formation of alginate beads with entrapped bacterial cells. The long-term activity of BL21_MlrA in comparison with wild Sphingomonas strain confirmed much higher potential of the modified bacteria. Immobilization in alginate allowed to form beads with high activity toward MC. A column packed with alginate entrapped cells eliminated MC efficiently from contaminated freshwater. These promising results will broaden the perspective of practical application of microorganisms in bioremediation of freshwater.

Index Terms—Alginate, biodegradation, immobilization, microcystin.

B. Wladyka is with Department of Analytical Biochemistry, Jagiellonian University, Krakow, Poland and Malopolska Centre of Biotechnology, Kraków, Poland (e-mail:
M. Wasylewski is with Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland (e-mail:


Cite:Dariusz Dziga, Magdalena Sworzen, Benedykt Wladyka, and Marcin Wasylewski, "," International Journal of Environmental Science and Development vol. 4, no. 4, pp. 360-364 , 2013.