General Information
    • ISSN: 2010-0264 (Print)
    • Abbreviated Title: Int. J. Environ. Sci. Dev.
    • Frequency: Monthly
    • DOI: 10.18178/IJESD
    • Editor-in-Chief: Prof. Richard Haynes
    • Executive Editor: Ms. Nancy Y. Liu
    • Abstracting/ Indexing: Scopus (Since 2019), Chemical Abstracts Services (CAS), EBSCO, CABI, Ulrich Periodicals Directory, Electronic Journals Library, Crossref, ProQuest.
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The University of Queensland, Australia
It is my honor to be the editor-in-chief of IJESD. The journal publishes good papers in the field of environmental science and development.
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IJESD 2010 Vol.1(1): 10-14 ISSN: 2010-0264
DOI: 10.7763/IJESD.2010.V1.3

Somatic Embryogenesis and Micropropagation in Teasle Gourd

Mohammad Abdul Karim 1+ and Shorif Uddin Ahmed 1

Abstract—In vitro somatic embryogenesis and subsequent plant regeneration was achieved in callus cultures derived from internode, node, shoot tip, petiole and leaf explant of teasle gourd plant on semi-solid Murashige and Skoog (MS) basal salts and growth regulators supplemented with 1.0mgl-1 BAP, 0.1mgl-1 NAA and 30 g l-1 (w/v) sucrose. Somatic embryos proliferated rapidly by somatic embryogenesis after 4 weeks. The embyogenic callus germinated on MS salts and growth regulators supplemented with 1.0mgl-1 BAP and 0.1mgl-1 NAA. The embryo-derived plantlets were transfered on half MS media with 0.3 mgl-1 IBA and sufficient rooting was achieved. Plantlets were acclimatized in the controlled environment.

Index Terms—Callus culture, Explant, Somatic embryogenesis & Teasle Gourd.

Professor, 1 Ph.D Student, Dept. of Crop Botany, Bangladesh Agricultural University, Mymensingh-2202, BANGLADESH


Cite: Mohammad Abdul Karim and Shorif Uddin Ahmed, "Somatic Embryogenesis and Micropropagationin Teasle Gourd," International Journal of Environmental Science and Development vol. 1, no. 1, pp. 10-14, 2010.

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