Abstract—In vitro somatic embryogenesis and subsequent plant regeneration was achieved in callus cultures derived from internode, node, shoot tip, petiole and leaf explant of teasle gourd plant on semi-solid Murashige and Skoog (MS) basal salts and growth regulators supplemented with 1.0mgl-1 BAP, 0.1mgl-1 NAA and 30 g l-1 (w/v) sucrose. Somatic embryos proliferated rapidly by somatic embryogenesis after 4 weeks. The embyogenic callus germinated on MS salts and growth regulators supplemented with 1.0mgl-1 BAP and 0.1mgl-1 NAA. The embryo-derived plantlets were transfered on half MS media with 0.3 mgl-1 IBA and sufficient rooting was achieved. Plantlets were acclimatized in the controlled environment.
Index Terms—Callus culture, Explant, Somatic embryogenesis & Teasle Gourd.
Professor, 1 Ph.D Student, Dept. of Crop Botany, Bangladesh Agricultural University, Mymensingh-2202, BANGLADESH
Cite: Mohammad Abdul Karim and Shorif Uddin Ahmed, "Somatic Embryogenesis and Micropropagationin Teasle Gourd," International Journal of Environmental Science and Development vol. 1, no. 1, pp. 10-14, 2010.