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IJESD 2013 Vol.4(5): 525-528 ISSN: 2010-0264
DOI: 10.7763/IJESD.2013.V4.407

Isolation and Molecular Identification of an Aerobic Denitrifier

Hongyu Wang, Kai Yang, Bin Ji, and Yu Jiang

Abstract—Six bacteria strains of which total nitrogen removal efficiency is over 70% were isolated from the activated sludge after enrichment culture. The strain Z31 with higher nitrogen removal was selected and its characteristic of aerobic denitrification was confirmed by the nitrogen element track. The results showed that the nitrate in the culture could be efficiently removed by strain Z31 and the nitrate nitrogen removal rate was up to above 95%. There was obvious nitrite accumulation during the denitrification process. Moreover, medium pH was increased and medium ORP was decreased as a result of denitrification. According to the morphological observation, physiological biochemical test and sequence analysis of the 16S rDNA, strain Z31 was identified as Pseudomonas stutzeri. And the phylogentic position of the strain was performed based on the phylogenetic tree.

Index Terms—Aerobic denitrifier, biological nitrogen removal, nitrate wastewater, Pseudomonas stutzeri.

Hongyu Wang is with School of Civil Engineering, Wuhan University, Wuhan 430070, P. R. China (email: hywang96@126.com).

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Cite:Hongyu Wang, Kai Yang, Bin Ji, and Yu Jiang, "Isolation and Molecular Identification of an Aerobic Denitrifier," International Journal of Environmental Science and Development vol. 4, no. 5, pp. 525-528, 2013.

General Information

  • ISSN: 2010-0264 (Print)
  • Abbreviated Title: Int. J. Environ. Sci. Dev.
  • Frequency: Bimonthly
  • DOI: 10.18178/IJESD
  • Editor-in-Chief: Prof. Richard Haynes
  • Executive Editor: Ms. Nancy Y. Liu
  • Indexing: Scopus (CiteScore 2021: 0.8), Google Scholar, CNKI, ProQuest, EBSCO, etc. 
  • E-mail: ijesd@ejournal.net