—Six bacteria strains of which total nitrogen
removal efficiency is over 70% were isolated from the activated
sludge after enrichment culture. The strain Z31 with higher
nitrogen removal was selected and its characteristic of aerobic
denitrification was confirmed by the nitrogen element track.
The results showed that the nitrate in the culture could be
efficiently removed by strain Z31 and the nitrate nitrogen
removal rate was up to above 95%. There was obvious nitrite
accumulation during the denitrification process. Moreover,
medium pH was increased and medium ORP was decreased as
a result of denitrification. According to the morphological
observation, physiological biochemical test and sequence
analysis of the 16S rDNA, strain Z31 was identified as
Pseudomonas stutzeri. And the phylogentic position of the
strain was performed based on the phylogenetic tree.
—Aerobic denitrifier, biological nitrogen
removal, nitrate wastewater, Pseudomonas stutzeri.
Hongyu Wang is with School of Civil Engineering, Wuhan University,
Wuhan 430070, P. R. China (email: firstname.lastname@example.org).
Cite:Hongyu Wang, Kai Yang, Bin Ji, and Yu Jiang, "Isolation and Molecular Identification of an Aerobic Denitrifier," International Journal of Environmental Science and Development vol. 4, no. 5, pp. 525-528, 2013.