doi: 10.18178/ijesd.2019.10.12.1213
Synergism and Antagonism among Indigenous Hydrolytic Bacteria from Biomedical Wastes for the Generation of Bacterial Consortium Used as Bioremediation Agent
Stalis Norma Ethica1
,
Rifki Muslim2 ,
RM Bagus Irawan Widyawardhana3 ,
Akbar Firmansyah4 ,
Sakti Imam Muchlissin5 ,
Sri Darmawati1
- 1the Medical Laboratory Technology Study Program, Faculty of Nursing and Health Sciences, Universitas Muhammadiyah Semarang, 50273, Indonesia. They are now with the Magister Program of Medical Laboratory Science, Universitas Muhammadiyah Semarang, 50273, Indonesia
- 2Medical Faculty, Universitas Muhammadiyah Semarang, 50273, Indonesia
- 3Mechanical Engineering Study program, Faculty of Engineering, Universitas Muhamamdiyah Semarang, 50273, Indonesia
- 4Faculty of Marine Science and Fisheries, Universitas Diponegoro, Semarang, 50275, Indonesia
- 5Magister of Marine Sciences Study Program, Universitas Diponegoro, Semarang, 50275, Indonesia
Abstract
The key steps to develop bioremediation agent from indigenous group of hydrolytic bacteria include isolation of the bacteria from polluted site followed by evaluation on pathogenicity levels and interactions among the isolated strains. This study aimed to evaluate synergism and antagonism between non-pathogenic and pathogenic groups of hydrolytic bacteria isolated from liquid biomedical wastes of hospitals in Semarang. Interactions among indigenous, non-pathogenic, hydrolytic bacterial isolates i.e. <em>Bacillus velezensis</em> R1.3, <em>B. amyloliquefaciens</em> R1.6,<em> B. amyloliquefaciens</em> R1.14, <em>B. velezensis</em> R1.16, <em>B.</em> <em>licheniformis</em> R2.5, and <em>B. amyloliquefaciens</em> R2.9 and with 20 other pathogenic ones were evaluated using overlay and cross streak methods. Observation was based on the formation of inhibition zone as evidence of antagonistic interaction and the absence of this zone as proof of synergic interaction. As results, the 6 non-pathogenic isolates are all synergic among each other, yet antagonistic against most of the 20 pathogenic ones. As conclusion, the obtained 6 non-pathogenic isolates could be mixed as bacterial consortium and used as major component of bioremediation agent of liquid hospital waste.
Keywords
- Bioremediation agent
- biomedical waste
- hydrolytic bacteria
- Semarang hospital
How to Cite
Stalis Norma Ethica, Rifki Muslim, RM Bagus Irawan Widyawardhana, Akbar Firmansyah, Sakti Imam Muchlissin, and Sri Darmawati, "Synergism and Antagonism among Indigenous Hydrolytic Bacteria from Biomedical Wastes for the Generation of Bacterial Consortium Used as Bioremediation Agent," International Journal of Environmental Science and Development, vol. 10, no. 12, pp. 440-444, 2019. https://doi.org/10.18178/ijesd.2019.10.12.1213
Copyright & License
Copyright © 2019 by the authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).