IJESD 2016 Vol.7(5): 351-354 ISSN: 2010-0264
DOI: 10.7763/IJESD.2016.V7.798
DOI: 10.7763/IJESD.2016.V7.798
Isolation and Characterization of Atrazine-Degrading Xanthomonas sp. ARB2 and Its Use in Bioremediation of Contaminated Soils
Sopid Sawangjit
Abstract—Thai degrading-bacteria, strain ARB2, was isolated from sugarcane field soils in Ratchaburi province of Thailand. Strain ARB2 was gram-negative bacteria, rod shape in pair and a single cell. The genomic DNA was extracted following the standard protocol for bacterial genomic DNA preparations. The partial 16S rDNA genes were amplified by polymerase chain reaction (PCR) using the universal primers of 16S rDNA gene. Sequence analysis of the PCR product indicated that the 16S rRNA gene in strain ARB2 was ranging from 89-91% identical to the same region in Xanthomonas sp. and were named Xanthomonas sp. ARB2. Xanthomonas sp. ARB2 was capable of degrading 300 mg L-1 and 100 mg kg-1 of atrazine in mineral salts liquid medium and soil at 81% and 62% in 7 days, respectively. GC-MS analysis detected the formation of two metabolites, deethylatrazine (DEA) and deisopropylatrazine (DIA) during the process of degradation of atrazine.
Index Terms—Atrazine, biodegradation, bioremediation, contaminated soil.
Sopid Sawangjit is with the Department of Biotechnology, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok, CO 13000, Thailand (e-mail: sopid.sa@ssru.ac.th, sawangjit.s@hotmail.com).
Index Terms—Atrazine, biodegradation, bioremediation, contaminated soil.
Sopid Sawangjit is with the Department of Biotechnology, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok, CO 13000, Thailand (e-mail: sopid.sa@ssru.ac.th, sawangjit.s@hotmail.com).
Cite: Sopid Sawangjit, "Isolation and Characterization of Atrazine-Degrading Xanthomonas sp. ARB2 and Its Use in Bioremediation of Contaminated Soils," International Journal of Environmental Science and Development vol. 7, no. 5, pp. 351-354, 2016.
General Information
- ISSN: 2010-0264 (Print); 2972-3698 (Online)
- Abbreviated Title: Int. J. Environ. Sci. Dev.
- Frequency: Bimonthly
- DOI: 10.18178/IJESD
- Editor-in-Chief: Prof. Richard Haynes
- Managing Editor: Ms. Cherry L. Chen
- Indexing: Scopus (CiteScore 2022: 1.4), Google Scholar, CNKI, ProQuest, EBSCO, etc.
- E-mail: ijesd@ejournal.net
List group item headingWed
Some placeholder content in a paragraph below the heading and date.
List group item headingTues
Some placeholder content in a paragraph below the heading and date.
List group item headingMon
Some placeholder content in a paragraph below the heading and date.
List group item headingWed
Some placeholder content in a paragraph below the heading and date.
List group item headingTues
Some placeholder content in a paragraph below the heading and date.
List group item headingMon
Some placeholder content in a paragraph below the heading and date.
List group item headingWed
Some placeholder content in a paragraph below the heading and date.
List group item headingTues
Some placeholder content in a paragraph below the heading and date.
List group item headingMon
Some placeholder content in a paragraph below the heading and date.
List group item headingWed
Some placeholder content in a paragraph below the heading and date.
List group item headingTues
Some placeholder content in a paragraph below the heading and date.
List group item headingMon
Some placeholder content in a paragraph below the heading and date.
Call for Papers
When submitting papers for potential publication in IJESD, please submit an original editable file in .doc style file. All figures, tables, and equations, etc., should be embedded into the original file.