Abstract—In this investigation of Iophon Laevistylus, collected from Faro Island in Persian Gulf, was extracted of polar and nonpolar contents using the method of Bligh & Dyer. Methanolic and diethyletter were used as extraction solvents, after removal of the solvents, the GC/MS spectra of the fraction were obtained. Identification of constituents was made by comparison of their masss pectra and retention indices (RI) with those given in the literature and authenic samples. 19 components respecting 95% were identified carbohydrate. In vitro antibacterial screening, against selected clinical isolates of bacteria was conducted in this study. Methanolic and diethy letter extracts demonstrated activity against Escherichia coli and Bacillus subtilis microbes tested. MIC and MBC were determined using Bacterial Broth Dilution Methods. The extracts showing good antimicrobial activity are undergoing further analysis to identify the active constituents.
Index Terms—Iophon Laevistylus, MIC (minimum inhibitory concentration), MBC(minimum bacterial concentrate), Antibacterial activity and Persian Gulf.
Melika Nazemi is with the Iranian Fisheries Research Organization, Tehran, Iran, P. O. Box 14155-6116 (corresponding author, phone: +98-912- 3612456; fax: +98-21-66514381; e-mail: email@example.com).
Zhaleh Khoshkhoo is with the Department of Fisheries, Facuty of Natural Resources, North Tehran Branch of Islamic Azad University, Tehran, Iran(e-mail: firstname.lastname@example.org).
Abbasali Motalebi is with Iranian Fisheries Research Organization, Tehran, Iran, P. O. Box 14155-6116. (e-mail: email@example.com).
Hosseinali Karimi Firuzjaii is with Islamic Azad University of Rudehen Branch, Rudehen, Tehran, Iran. (e-mail: firstname.lastname@example.org).
Fatemeh Pishehvarzad is with Guilan University, Iran (e-mail:Andishmand45@yahoo.com)
Cite: Melika Nazemi, Zhaleh Khoshkhoo, Abbasali Motalebi, Hosseinali Karimi Firozjaee and Fatemeh Pishehvarzad, "Identification nonpolar component and antibacterial activities of Iophon laevistylus from Persian Gulf," International Journal of Environmental Science and Development vol. 1, no. 2, pp. 107-110, 2010.