IJESD 2015 Vol.6(6): 439-444 ISSN: 2010-0264
DOI: 10.7763/IJESD.2015.V6.633
DOI: 10.7763/IJESD.2015.V6.633
Kinetic Study of Immobilized Whole-Cell Lipase Rhizopus Oryzae as Biocatalyst in Biodiesel Synthesis through Non-Alcohol Route
H. Hermansyah, M. B. Faiz, and R. Arbianti
Abstract—Utilizing whole-cell biocatalyst is a potential way to reduce catalyst cost in biodiesel production using lipase as catalyst. Whole-cell of Rhizopus oryzae was cultivated by one-step and two-step method and was immobilized on Biomass Support Particles (BSPs) and chitosan-TPP bead. Immobilized whole-cells on BSPs produce 11% (one-step) and 12% (two-step) FAME yield. While, FAME yield produced by immobilized whole-cell in chitosan-TPP beads are 23% (one-step) and 22% (two-step). Kinetic model based Michaelis-Menten used was found to fit fairly the substrate and product concentration profile. Value of Km and Vmax for R. oryzae whole-cell immobilized on BSP are 4 mole L-1, 0.05 mole L-1 h-1 (one-step) and 3 mole L-1, 0.04 mol L-1 h-1 (two-step). While, for immobilized whole-cell in chitosan-TPP bead, the values are 0.3 mole L-1, 0.01 mole L-1 h-1 and 0.2 mole L-1, 0.008 mole L-1 h-1 for single-step and two-step respectively.
Index Terms—Biodiesel, immobilization, Michaelis-Menten, whole-cell.
H. Hermansyah, M. B. Faiz, and R. Arbianti are with Universitas Indonesia, Depok 16424, Indonesia (e-mail: heri@che.ui.ac.id, merisa.faiz@ui.ac.id, arbianti@che.ui.ac.id).
Index Terms—Biodiesel, immobilization, Michaelis-Menten, whole-cell.
H. Hermansyah, M. B. Faiz, and R. Arbianti are with Universitas Indonesia, Depok 16424, Indonesia (e-mail: heri@che.ui.ac.id, merisa.faiz@ui.ac.id, arbianti@che.ui.ac.id).
Cite:H. Hermansyah, M. B. Faiz, and R. Arbianti, "Kinetic Study of Immobilized Whole-Cell Lipase Rhizopus Oryzae as Biocatalyst in Biodiesel Synthesis through Non-Alcohol Route," International Journal of Environmental Science and Development vol. 6, no. 6, pp. 439-444, 2015.
General Information
- ISSN: 2010-0264 (Print); 2972-3698 (Online)
- Abbreviated Title: Int. J. Environ. Sci. Dev.
- Frequency: Bimonthly
- DOI: 10.18178/IJESD
- Editor-in-Chief: Prof. Richard Haynes
- Managing Editor: Ms. Cherry L. Chen
- Indexing: Scopus (CiteScore 2022: 1.4), Google Scholar, CNKI, ProQuest, EBSCO, etc.
- E-mail: ijesd@ejournal.net
List group item headingWed
Some placeholder content in a paragraph below the heading and date.
List group item headingTues
Some placeholder content in a paragraph below the heading and date.
List group item headingMon
Some placeholder content in a paragraph below the heading and date.
List group item headingWed
Some placeholder content in a paragraph below the heading and date.
List group item headingTues
Some placeholder content in a paragraph below the heading and date.
List group item headingMon
Some placeholder content in a paragraph below the heading and date.
List group item headingWed
Some placeholder content in a paragraph below the heading and date.
List group item headingTues
Some placeholder content in a paragraph below the heading and date.
List group item headingMon
Some placeholder content in a paragraph below the heading and date.
List group item headingWed
Some placeholder content in a paragraph below the heading and date.
List group item headingTues
Some placeholder content in a paragraph below the heading and date.
List group item headingMon
Some placeholder content in a paragraph below the heading and date.
Call for Papers
When submitting papers for potential publication in IJESD, please submit an original editable file in .doc style file. All figures, tables, and equations, etc., should be embedded into the original file.