—The application of crude protease of Aspergillus oryzae to suppress the hatchability of Pomacea canaliculata eggs is highlighted. The enzyme was produced through solid state fermentation (SSF) using cassava, which is cheap and abundantly available. It was found that the enzyme suppressed the hatchability of P. canaliculata eggs as much as 30% and 20% at 20.8U/ml enzyme for 3 and 9 days old eggs respectively. The ANOVA result revealed that the hatchability was significantly affected by both protease concentration as well as egg’s age. The LC50 for 3 and 9 days old eggs were 3.29 U/ml and 3.54 U/ml, respectively; while LC95 for 3 and 9 days old eggs were 19.11 U/ml and 20.09 U/ml respectively. It is proposed that the crude protease propagated from SSF using locally available and cheap material should be considered as an alternative biopesticide to suppress the hatchability of P. canaliculata eggs.
—Aspergillus oryzae, pomacea canaliculata, hatchability, eggs.
The authors are with the School of Bioprocess Engineering, University Malaysia Perlis, 02600 Arau, Perlis, Malaysia (e-mail: firstname.lastname@example.org, email@example.com).
Cite:Noor Hasyierah Mohd Salleh and Dachyar Arbain, "Application of Crude Protease from Cheap and Local Raw Material as a Biopesticide for the Disruption of Pomacea Canaliculata Eggs," International Journal of Environmental Science and Development vol. 6, no. 4, pp. 275-278, 2015.