General Information
    • ISSN: 2010-0264
    • Frequency: Bimonthly (2010-2014); Monthly (Since 2015)
    • DOI: 10.18178/IJESD
    • Editor-in-Chief: Prof. Richard Haynes
    • Executive Editor: Ms. Nancy Y. Liu
    • Abstracting/ Indexing: Chemical Abstracts Services (CAS), CABI, DOAJ, Ulrich Periodicals Directory, Engineering & Technology Digital Library, Electronic Journals Library, Crossref, ProQuest.
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The University of Queensland, Australia
It is my honor to be the editor-in-chief of IJESD. The journal publishes good papers in the field of environmental science and development.
IJESD 2015 Vol.6(4): 241-245 ISSN: 2010-0264
DOI: 10.7763/IJESD.2015.V6.598

Bio-Enrichment of Waste Crude Oil Polluted Soil: Amended with Bacillus 139SI and Organic Waste

Arezoo Dadrasnia and Salmah Binti Ismail
Abstract—Biodegradation of waste crude oil contaminated soil amended by Bacillus 139SI and used tea leaf amendments was investigated to determine the rate of hydrocarbon remediation. Previously, Bacillus 139SI was isolated from an agricultural soil in the Serdang agricultural center, Malaysia. Within 60 days, 14% oil loss was recorded in unamended polluted autoclaved soil, while waste crude oil disappeared more rapidly in the soil amendment with both strain and organic waste, recorded above 89%. Utilizing bacteria counts were significantly higher in all amended treatments comparing to control soil. Dehydrogenase activity in soil was markedly enhanced by the application of amendments. Waste crude oil composition monitored by GC/FID indicated complete degradation of n-C9–C25. First-order kinetic model revealed that organic waste and strain were the best of treatments, with biodegradation rate constant of 0.17day-1and half life of 4 days. The results showed there is potential for tea leaf and Bacillus 139SI to enhance biodegradation of waste crude oil contaminated soil.

Index Terms—Bioaugmentation, biostimulation, microbial consortium, organic waste, waste crude oil.

The authors are with the Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia (e-mail:,


Cite:Bio-Enrichment of Waste Crude Oil Polluted Soil: Amended with Bacillus 139SI and Organic Waste, "Arezoo Dadrasnia and Salmah Binti Ismail," International Journal of Environmental Science and Development vol. 6, no. 4, pp. 241-245, 2015.

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