Abstract—In this study, different combinations of benzylaminopurine (BAP) and naphthalene acetic acid (NAA) were used to establish an efficient in vitro regeneration in two different types of explants: stem and shoot tip of seedlings obtained from in vitro seed germination of Passiflora edulis Sims var. edulis. The results obtained showed that BAP induced shoots while NAA induced roots. Scanning Electron Microscopy (SEM) studies were carried out to observe and differentiate the morphology of in vitro and in vivo grown Passiflora edulis Sims var. edulis. SEM techniques were done to observe the stomata and microcharacters of both the abaxial and adaxial surfaces of in vitro and in vivo leaves of Passiflora edulis Sims var. edulis.
Index Terms—In vitro, in vivo, Passiflora edulis Sims var. edulis, regeneration, Scanning Electron Microscopy (SEM).
All the authors are with the Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia (e-mail: firstname.lastname@example.org, email@example.com, firstname.lastname@example.org).
Cite:Rubashiny Veeramohan, Noorma Wati Haron, and Rosna Mat Taha, "Scanning Electron Microscopy Studies and In Vitro Regeneration of Passiflora edulis Sims var. edulis for Conservation," International Journal of Environmental Science and Development vol. 4, no. 5, pp. 586-590, 2013.