Abstract—Antimicrobial efficiency of extracts derived from Trifolium pratense (in vivo and in vitro grown plants, including callus) were examined using ethanol and methanol as solvents and tested against four bacterial pathogens (two gram negative and two gram positive) and three fungal pathogens. The antimicrobial activity of the methanol extract was found to give higher inhibition zone when compared with ethanol extract. Among the callus, in vitro and in vivo grown plants, the callus extract showed better antimicrobial activity, thus revealing a new potential use of callus of Trifolium pretense.
Index Terms—Antimicrobial, Callus, in vitro, Trifolium pratense
The authors are with Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia (e-mail: firstname.lastname@example.org, email@example.com, firstname.lastname@example.org, mohajer.ae@ gmail.com, email@example.com, firstname.lastname@example.org).
Cite:Arash Khorasani Esmaeili, Rosna Mat Taha, Behrooz Banisalam, Sadegh Mohajer, and Noor Zalina Mahmood, "Antimicrobial Activities of Extracts Derived from in vivo and in vitro Grown Trifolium pratense (Red clover)," International Journal of Environmental Science and Development vol. 4, no. 5, pp. 475-478, 2013.