Abstract

Using the constructed wetland of Yan Huang ditch of Qihe County treat wastewater, and the real-time Polymerase Chain Reaction(PCR) technology was used to detect the water abundance of nitrification functional gene amoA and the abundance of denitrification function gene nirS, nirK and nosZ. The results showed that the whole abundance of amoA was low. At each sampling point, nosZ was the highest abundance gene, and nirS had the same trend with nirK, and the abundance of nirS was higher than the abundance of nirK. The study showed that the removal rate of chemical oxygen demand (COD) was relatively high, but the removal rate of ammonia nitrogen was lower, and the effect of nitrogen removal was not obvious, and the end product of denitrification was nitrogen. The content of ammonium nitrogen (NH4+-N) in water had significant negative correlation with the abundance of amoA, but the content of nitrate nitrogen (NO3--N) had no significant correlation with the abundance of nirS, nirK and nosZ, which indicated that there were other nitrogen removal mechanisms in addition to nitrification-denitrification.