doi: 10.7763/IJESD.2012.V3.186
Frozen-thawed Sperm Motility Characteristics of African Catfish (Clarias gariepinus) by Using Glycerol or DMSO Based Extender
Noor Azlina Kamaruding1
,
Wan Khadijah Wan Embong2 ,
Ramli Bin Abdullah2
- 1the Department of Biological Sciences, Faculty of Science and Technology, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, MALAYSIA
- 2Animal Biotechnology-Embryo Laboratory (ABEL), Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Abstract
The objective of this study was to evaluate the efficacy of Tris-Citrate Acid Yolk Extender and Fish Ringer Extender on sperm motility characteristics in African Catfish with special focus on equilibration durations (120, 140 or 160 min), vapor temperatures (-80, -90 or -100oC) and vapor exposure durations (5, 10 or 15 min) as well as the fish body weights. Combination of DMSO (10%) in FRE extender showed significantly the highest values of respective post-thawed total motility and progressive motility (73.52±1.35% and 18.37±0.61%) as compared to the three molarities of glycerol (0.5, 1.0 and 2.0 M) in TCAYE extender (32.27±2.05 and 3.75±0.41; 24.50±1.81 and 2.63±0.29; 26.74±2.14 and 2.45±0.37). It is suggested that FRE is a preferred extender to TCAYE to freeze the African Catfish sperm.
Keywords
- Equilibration
- extender
- sperm cryopreservation
- vapor exposure
How to Cite
Noor Azlina Kamaruding, Wan Khadijah Wan Embong, and Ramli Bin Abdullah, "Frozen-thawed Sperm Motility Characteristics of African Catfish (Clarias gariepinus) by Using Glycerol or DMSO Based Extender," International Journal of Environmental Science and Development, vol. 3, no. 1, pp. 49-55, 2012. https://doi.org/10.7763/IJESD.2012.V3.186
Copyright & License
Copyright © 2012 by the authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).