Abstract—The objective of this study was to evaluate the efficacy of Tris-Citrate Acid Yolk Extender and Fish Ringer Extender on sperm motility characteristics in African Catfish with special focus on equilibration durations (120, 140 or 160 min), vapor temperatures (-80, -90 or -100oC) and vapor exposure durations (5, 10 or 15 min) as well as the fish body weights. Combination of DMSO (10%) in FRE extender showed significantly the highest values of respective post-thawed total motility and progressive motility (73.52±1.35% and 18.37±0.61%) as compared to the three molarities of glycerol (0.5, 1.0 and 2.0 M) in TCAYE extender (32.27±2.05 and 3.75±0.41; 24.50±1.81 and 2.63±0.29; 26.74±2.14 and 2.45±0.37). It is suggested that FRE is a preferred extender to TCAYE to freeze the African Catfish sperm.

Index Terms—Equilibration, extender, sperm cryopreservation, vapor exposure.

Noor Azlina Kamaruding is with the Department of Biological Sciences, Faculty of Science and Technology, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, MALAYSIA (E-mail: noor.azlina@umt.edu.my).
Wan Khadijah Wan Embong and Ramli Bin Abdullah are with Animal Biotechnology-Embryo Laboratory (ABEL), Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MALAYSIA (E-mail: wkhadi@um.edu.my; ramli@um.edu.my).

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Cite: Noor Azlina Kamaruding, Wan Khadijah Wan Embong, and Ramli Bin Abdullan, "Frozen-thawed Sperm Motility Characteristics of African Catfish (Clarias gariepinus) by Using Glycerol or DMSO Based Extender," International Journal of Environmental Science and Development vol. 3, no. 1, pp. 49-55, 2012.