IJESD 2023 Vol.14(3): 180-184
doi: 10.18178/ijesd.2023.14.3.1431
doi: 10.18178/ijesd.2023.14.3.1431
Investigating the Ligninolytic Potential of Bacteria Isolated from a Decayed Wood Sample in Egypt
Prince Hotor*, Ahmed Hassanin, Mitsuo Yamamoto, Yukie Saito, and Ahmed Abdel-Mawgood
Manuscript received March 21, 2022; revised May 3, 2022; accepted June 29, 2022.
Abstract—Lignin is a natural polyphenol material found in
complex with cellulose and hemicellulose in every terrestrial
and some aquatic plant species. They are recalcitrant and
prevent easy access to cellulose; an energy-rich, versatile,
promising biomaterial for several eco-friendly and cheap
products. The lignin-degrading potential of microbes isolated
from degraded wood in an environment in Egypt was
investigated. In this study, four bacteria were screened using a
methylene blue decolorization test, growth on media with lignin
as carbon source, and cellulolytic screening using CMC-Congo
red test. The screened isolates were used to treat sorghum stem
stalks to ascertain the level of degradation of the lignin content.
The four bacterial isolates were positive to the decolorization of
methylene blue with percentage decolorization: (23%, 8%, 34%,
and 24%) after 24 hrs. respectively. Similarly, the four isolates
were able to grow on minimal salt media with lignin as carbon
source. The optical densities values measured ranged from
(0.030–0.165, −0.040–0.080, 0.040–0.121, −0.039–0.054) after
12 h for the four bacterial isolates, respectively. Also, the four
bacterial isolates showed negative results for the cellulase test.
FT-IR spectrum for the bacterial-treated sorghum stalks
revealed a reduction in peaks at wavelengths indicative of lignin
functional groups. Similarly, proximate analysis of the
lignocellulose content after treatment recorded were extractives
(E) (9, 9, 13.5, 9.5, and 10) %, hemicellulose content (H) (29.5,
26.0, 31, 26, and 30) %, Klason lignin (L) (39, 31, 33, 24, and
26) %, and cellulose (C) (22.2, 33.5, 21.3, 40.5, and 33.5) % for
control and the four bacterial isolates, respectively. The
p-values estimated from the average values for the extractive
and hemicellulose content were not significantly different,
whiles that of Lignin and Cellulose was significantly different.
The four bacterial isolates showed potential for having
ligninolytic enzymes for lignin depolymerization.
Index Terms—Depolymerization, lignin, decolorization of methylene blue, Kraft lignin, ligninolytic ability
Prince Hotor and Ahmed Abdel-Mawgood are with the Department of Biotechnology, Egypt Japan University of Science and Technology, New Borg El-Arab, Alexandria, Egypt.
Ahmed Hassanin is with the Department of Materials Science and Engineering, Egypt Japan University of Science and Technology, New Borg El-Arab, Alexandria, Egypt. He is also with Department of Textile Engineering, Alexandria University, Alexandria, Egypt.
Mitsuo Yamamoto is with Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-Ku, Tokyo, Japan.
Yukie Saito is with Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-Ku, Tokyo, Japan.
*Correspondence: prince.hotor@ejust.edu.eg (P.H.)
Index Terms—Depolymerization, lignin, decolorization of methylene blue, Kraft lignin, ligninolytic ability
Prince Hotor and Ahmed Abdel-Mawgood are with the Department of Biotechnology, Egypt Japan University of Science and Technology, New Borg El-Arab, Alexandria, Egypt.
Ahmed Hassanin is with the Department of Materials Science and Engineering, Egypt Japan University of Science and Technology, New Borg El-Arab, Alexandria, Egypt. He is also with Department of Textile Engineering, Alexandria University, Alexandria, Egypt.
Mitsuo Yamamoto is with Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-Ku, Tokyo, Japan.
Yukie Saito is with Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-Ku, Tokyo, Japan.
*Correspondence: prince.hotor@ejust.edu.eg (P.H.)
Cite: Prince Hotor*, Ahmed Hassanin, Mitsuo Yamamoto, Yukie Saito, and Ahmed Abdel-Mawgood, "Investigating the Ligninolytic Potential of Bacteria Isolated from a Decayed Wood Sample in Egypt," International Journal of Environmental Science and Development vol. 14, no. 3, pp. 180-184, 2023.
Copyright © 2023 by the authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).General Information
- ISSN: 2010-0264 (Print); 2972-3698 (Online)
- Abbreviated Title: Int. J. Environ. Sci. Dev.
- Frequency: Bimonthly
- DOI: 10.18178/IJESD
- Editor-in-Chief: Prof. Richard Haynes
- Managing Editor: Ms. Cherry L. Chen
- Indexing: Scopus (CiteScore 2022: 1.4), Google Scholar, CNKI, ProQuest, EBSCO, etc.
- E-mail: ijesd@ejournal.net
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