International Journal of Environmental Science and Development

Abstract—To enhance the recovery rateofcellulase immobilization, various factors (enzyme dosage, temperature, pH and glutaraldehyde concentration) that affect the immobilization process were investigated and evaluated using response surface methodology. The exact effect of each factor was successfully simulated through a Box-Behnken design. The results showed these factors had significant liner, quadratic and interactive effects on recovery rate (p<0.05). The predicted optimal condition for this immobilization was an enzyme dosage (chitosan-to-enzyme ratio) 9.3, a temperature of 30.6 °C, a pH value of 5.3 and aConcentration of glutaraldehyde of 0.14% (m/V). The validation experiment showed the recovery rate of cellulase in this condition was 68.5%, which was in accordance with the predicted value 68.3%. The immobilized cellulase was recycled and reused in the cellulose hydrolysis process for five times and reached over 85% of the free enzyme hydrolysis efficiency.

Index Terms—Cellulase immobilization, cellulose hydrolysis, Recovery rate, response surface methodology.

The authors are with School of Environmental Science and Engineering, Shanghai Jiao Tong University, No.800 Dongchuan Road, Shanghai 200240, China (corresponding author: Y. Lin; e-mail: 249716843@qq.com, linyansjtu@126.com, fssz520@163.com, 1210208384@qq.com, 1196251048@qq.com).

[PDF]

Cite:Q. Zhang, Y. Lin, S. Shen, Z. Xing, and X. Ruan, "Simulation and Optimization on Cellulase Immobilization Using Response Surface Methodology," International Journal of Environmental Science and Development vol. 6, no. 9, pp. 664-667, 2015.