General Information
    • ISSN: 2010-0264
    • Frequency: Bimonthly (2010-2014); Monthly (Since 2015)
    • DOI: 10.18178/IJESD
    • Editor-in-Chief: Prof. Richard Haynes
    • Executive Editor: Ms. Nancy Y. Liu
    • Abstracting/ Indexing: Chemical Abstracts Services (CAS), CABI, Ulrich Periodicals Directory, Electronic Journals Library, Crossref, ProQuest.
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The University of Queensland, Australia
It is my honor to be the editor-in-chief of IJESD. The journal publishes good papers in the field of environmental science and development.
IJESD 2015 Vol.6(9): 664-667 ISSN: 2010-0264
DOI: 10.7763/IJESD.2015.V6.677

Simulation and Optimization on Cellulase Immobilization Using Response Surface Methodology

Q. Zhang, Y. Lin, S. Shen, Z. Xing, and X. Ruan
Abstract—To enhance the recovery rateofcellulase immobilization, various factors (enzyme dosage, temperature, pH and glutaraldehyde concentration) that affect the immobilization process were investigated and evaluated using response surface methodology. The exact effect of each factor was successfully simulated through a Box-Behnken design. The results showed these factors had significant liner, quadratic and interactive effects on recovery rate (p<0.05). The predicted optimal condition for this immobilization was an enzyme dosage (chitosan-to-enzyme ratio) 9.3, a temperature of 30.6 °C, a pH value of 5.3 and aConcentration of glutaraldehyde of 0.14% (m/V). The validation experiment showed the recovery rate of cellulase in this condition was 68.5%, which was in accordance with the predicted value 68.3%. The immobilized cellulase was recycled and reused in the cellulose hydrolysis process for five times and reached over 85% of the free enzyme hydrolysis efficiency.

Index Terms—Cellulase immobilization, cellulose hydrolysis, Recovery rate, response surface methodology.

The authors are with School of Environmental Science and Engineering, Shanghai Jiao Tong University, No.800 Dongchuan Road, Shanghai 200240, China (corresponding author: Y. Lin; e-mail:,,,,


Cite:Q. Zhang, Y. Lin, S. Shen, Z. Xing, and X. Ruan, "Simulation and Optimization on Cellulase Immobilization Using Response Surface Methodology," International Journal of Environmental Science and Development vol. 6, no. 9, pp. 664-667, 2015.

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