IJESD 2015 Vol.6(9): 664-667 ISSN: 2010-0264
DOI: 10.7763/IJESD.2015.V6.677
DOI: 10.7763/IJESD.2015.V6.677
Simulation and Optimization on Cellulase Immobilization Using Response Surface Methodology
Q. Zhang, Y. Lin, S. Shen, Z. Xing, and X. Ruan
Abstract—To enhance the recovery rateofcellulase
immobilization, various factors (enzyme dosage, temperature,
pH and glutaraldehyde concentration) that affect the
immobilization process were investigated and evaluated using
response surface methodology. The exact effect of each factor
was successfully simulated through a Box-Behnken design. The
results showed these factors had significant liner, quadratic and
interactive effects on recovery rate (p<0.05). The predicted
optimal condition for this immobilization was an enzyme dosage
(chitosan-to-enzyme ratio) 9.3, a temperature of 30.6 °C, a pH
value of 5.3 and aConcentration of glutaraldehyde of 0.14%
(m/V). The validation experiment showed the recovery rate of
cellulase in this condition was 68.5%, which was in accordance
with the predicted value 68.3%. The immobilized cellulase was
recycled and reused in the cellulose hydrolysis process for five
times and reached over 85% of the free enzyme hydrolysis
efficiency.
Index Terms—Cellulase immobilization, cellulose hydrolysis, Recovery rate, response surface methodology.
The authors are with School of Environmental Science and Engineering, Shanghai Jiao Tong University, No.800 Dongchuan Road, Shanghai 200240, China (corresponding author: Y. Lin; e-mail: 249716843@qq.com, linyansjtu@126.com, fssz520@163.com, 1210208384@qq.com, 1196251048@qq.com).
Index Terms—Cellulase immobilization, cellulose hydrolysis, Recovery rate, response surface methodology.
The authors are with School of Environmental Science and Engineering, Shanghai Jiao Tong University, No.800 Dongchuan Road, Shanghai 200240, China (corresponding author: Y. Lin; e-mail: 249716843@qq.com, linyansjtu@126.com, fssz520@163.com, 1210208384@qq.com, 1196251048@qq.com).
Cite:Q. Zhang, Y. Lin, S. Shen, Z. Xing, and X. Ruan, "Simulation and Optimization on Cellulase Immobilization Using Response Surface Methodology," International Journal of Environmental Science and Development vol. 6, no. 9, pp. 664-667, 2015.
General Information
- ISSN: 2010-0264 (Print); 2972-3698 (Online)
- Abbreviated Title: Int. J. Environ. Sci. Dev.
- Frequency: Bimonthly
- DOI: 10.18178/IJESD
- Editor-in-Chief: Prof. Richard Haynes
- Managing Editor: Ms. Cherry L. Chen
- Indexing: Scopus (CiteScore 2022: 1.4), Google Scholar, CNKI, ProQuest, EBSCO, etc.
- E-mail: ijesd@ejournal.net
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