Abstract—The presence of inhibitors such as hydroxymethyl furfural (HMF) and dissolved salts on the hydrolysate disrupt the growth of Saccharomyces cerevisiae in fermentation process. The objective of research was to increase the yield of ethanol in fermentation process through neutralization and detoxification of Eucheuma cottonii hydrolysate. Hydrolysate was neutralized and overliming with Ca(OH) 2 and NH 4 OH and continued by activated charcoal adsorption. The results showed that the highest content of reducing sugars was 11.34% (w/v) on neutralization with Ca(OH) 2 . The lowest content of HMF was 1.39 g/kg hydrolysate on overliming treatment with Ca(OH) 2 . Overliming treatment with Ca(OH) 2 was able to reduce levels of HMF up to 16.77%, while the treatment with NH 4 OH overliming contributed to 1.06% for the HMF reduction. The highest ethanol yield was 2.49% (v/v) through neutral ization with Ca(OH)2, while neutralizat ion with NH4OH treatment was 0.49% (v/v). The low yield of ethanol on treatment with NH4OH due to inhibition by HMF and high levels of salinity in the hydrolysate, 1.89 g/kg hydrolysate and 1600/00 respectively. The fermentation efficiency obtained at treatment by neutralization with Ca(OH)2 was 56.14%.
Index Terms—Bioethanol, Eucheuma cottonii, neutralization and detoxification.
The authors are with the Department of Agroindustrial Technology, Bogor Agricultural University, Indonesia (e-mail: email@example.com, firstname.lastname@example.org, email@example.com).
Cite:Jaini Fakhrudin, Dwi Setyaningsih, and Mulyorini Rahayuningsih, "Bioethanol Production from Seaweed Eucheuma cottonii by Neutralization and Detoxification of Acidic Catalyzed Hydrolysate," International Journal of Environmental Science and Development vol. 5, no. 5, pp. 455-458, 2014.