—This study examines fermentative hydrogen
production from untreated (raw) sludge and pretreated sludge
by sterilization treatment (15, 30, 45, and 60 min) as substrate
using mixed cultures in batch experiments under anaerobic
thermophilic conditions. Longer treatment time was found to be
highly effective for hydrolyzing organic matters in the sludge.
Soluble chemical oxygen demand (SCOD) of pretreated sludge
was 1.2 to 1.9-fold higher than that of untreated sludge.
Sterilization treatment was found to accelerate and increase
hydrogen production throughout the batch mode, but with no
measurable methane production. Pretreated (30 min) sludge
presented an optimal condition, resulting in maximum
hydrogen yield (25.1 ml H2/g-VS) and the highest hydrogen
content (60.0%). Under the same conditions, enhanced
hydrogen yield was 6.4-fold higher, which came with an
additional benefit of efficient VS removal over the use of
untreated sludge. This was attributed to destruction of solids in
sludge during the solubilization process. Present findings have
potential practical use in not only processes for efficient
hydrogen production via anaerobic fermentation but also in
—Sewage sludge, sterilization treatment,
hydrogen production, mixed cultures, anaerobic fermentation.
Mijung Kim, Randeep Rakwal, Chunguang Liu, and Zhenya Zhang are
with the Graduate School of Life and Environmental Sciences, University of
Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Sechang Oh is with the Graduate School of Comprehensive Human
Science, University of Tsukuba, Tsukuba, Ibaraki 305-8574, Japan.
Randeep Rakwal is also with the Organization for Educational Initiatives,
University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan.
Mijung Kim, Present address: National Institute of Advanced Industrial
Science & Technology (AIST), Tsukuba, Ibaraki 305-8562, Japan (e-mail:
Cite:Mijung Kim, Sechang Oh, Randeep Rakwal, Chunguang Liu, and Zhenya Zhang, "Simultaneous Organic Solids Disintegration and Fermentative Hydrogen Production by Pretreated Sludge Generated from Wastewater Treatment Plant," International Journal of Environmental Science and Development vol. 4, no. 6, pp. 617-623, 2013.