Abstract—Petroleum products leakages result in important
effects on the normal operation of circulating cooling water
system. However, relatively little research has been done to
explore effects of petroleum products leakages on biofilm
growth characteristics. In this study, diesel as the experimental
subject representing petroleum products, effects of diesel
addition on biofilm growth characteristics were investigated.
Increase of diesel addition led to biofilm EPS increase with
diesel concentration less than 200 mg/L, then EPS content were
kept relatively stable with diesel concentration more than 200
mg/L. Protein contents were found at relatively higher levels
than polysaccharides in the biofilm with diesel concentration
more than 200 mg/L. Expect for 100 mg/L diesel, increasing
diesel concentration enhanced biofilm detachment ratio with
respect to the control test. Although biofilm wet weight tended
to increase with diesel concentration rising from 0 to 1500 mg/L,
there was a marked reduction in lipid phosphorus content with
increase of diesel concentration from 200 to 1500 mg/L. The
reduction indicated that diesel was toxic to microbial
population in biofilm.
Index Terms—Circulating cooling water, leakage, diesel, biofilm, growth characteristics.
The authors are with the College of Chemical Engineering, China University of Petroleum, Qingdao 266555 (e-mail: firstname.lastname@example.org, email@example.com, firstname.lastname@example.org).
Cite:Fang Liu, Wei Yang, HuiYun Zhong, JinJin Lu, and Chaocheng Zhao, "Biofilm Growth Characteristics at Different Diesel Leakage Concentration," International Journal of Environmental Science and Development vol. 4, no. 2, pp. 111-115, 2013.