—Reliable methods are needed to measure and correlate bioavailable metals with aquatic toxicity. This research develops a method to measure bioavailable copper in estuarine waters using the cation exchange resin Chelex 100®. The Chelex 100® method performed consistently at copper concentrations from 195-495 μg/L when organic matter, pH and salinity were held constant. Varying salinity from 15-30 ppt did not affect measured bioavailable copper. As expected, an increase in pH from 4 to 8.5 and increase in NOM from 0 to 12.5 mg/L reduced measured bioavailable copper. Acute toxicity bioassays were performed with mysid shrimp (Americamysis bahia) and copper in the presence of NOM. At 20 ppt salinity, 48-hour LC50 dissolved copper concentrations were 200, 340, and 495 μg/L at 0, 12, and 24 mg/L NOM, respectively. The corresponding 48-hour LC50 values for bioavailable copper were nearly constant: 94, 98, and 105 μg/L Cu at 0, 12, and 24 mg/L NOM respectively. The consistency of the mysid shrimp LC50 values for bioavailable copper measured using the Chelex-100® method indicates that this method is appropriate for evaluating metal bioavailability in saline waters.
—Bioavailable, copper, salinity, shrimp, toxicity.
A. M. Dietrich and D. L. Gallagher are with the Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg VA 24060-0246 (e-mail: firstname.lastname@example.org; email@example.com).
N. Postlethwait is an Associate Project Manager at CH2M HILL, Tampa, FL 33607, (e-mail: firstname.lastname@example.org). The research was conducted when he was a graduate student at the Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg VA 24060-0246.
Cite:Andrea M. Dietrich, Niel Postlethwait, and Daniel L. Gallagher, "Quantifying Bioavailability and Toxicity of Copper to Americamysis bahia - Mysid Shrimp," International Journal of Environmental Science and Development vol. 4, no. 1, pp. 37-43, 2013.